Modelling G2019S-LRRK2 sporadic Parkinson’s disease in 3D midbrain organoids. Modelling G2019S-LRRK2 sporadic Parkinson’s disease in 3D midbrain organoids

Recent advances in generating 3 dimensional (3D) organoid systems from stem cells offer new possibilities for disease modeling. In this study, we generate isogenic 3D midbrain organoids with or without a Parkinson’s disease-associated LRRK2 G2019S mutation. LRRK2-G2019S midbrain organoids derived from LRRK2 targeted human iPSCs in vitro have LRRK2-associated sporadic Parkinson's disease phenotypes. Midbrain-like 3D organoids expressing LRRK2-G2019S showed dynamic changes in globle gene expression. Overall design: Affymetrix GeneChip Human Gene 1.0 ST Array was performed according to the manufacturer's protocol. Changes in the gene expression profile of 2D control, 2D LRRK2-G2019S, 3D midbrain organoids and LRRK2-G2019S 3D oragnoids