Transcriptomic changes in C3H10T1/2 cells exposed to lipid deprivation. Transcriptomic changes in C3H10T1/2 cells exposed to lipid deprivation

The avascular nature of cartilage makes it a unique tissue in the human body, but whether and how the absence of blood vessels regulates chondrogenesis remains unknown. Obstruction of vascular invasion during bone healing favours chondrogenic over osteogenic differentiation of skeletal progenitor cells, a process we observed to be driven by a decreased availability of extracellular lipids. To understand how lipid deprivation activates the chondrogenic programme, we profiled mRNA expression of C3H10T1/2 skeletal progenitors exposed to lipid deprivation for 1, 3 or 6 hours.