Skin multi-omics-based interactome analysis: Integrating the tissue and mucus exuded layer for a comprehensive understanding of the teleost mucosa functionality as model of study

In this study, we analyzed both together the epithelial tissue and the secreted mucus response using a holistic interactome-based multi-omics approach. The effect of the gilthead sea bream (Sparus aurata) skin mucosa to a dietary inclusion of spray-dried porcine plasma (SDPP) was evaluated. Fish (BWi = 10.6 ± 0.1 g, n = 400, mean ± standard deviation, SD) were fed for 95 days with a control diet (Diet C; equivalent to commercial diet containing 51% crude protein, 17% crude fat and 20.6 MJ/kg gross energy that fulfil the nutritional requirements of juvenile sea bream) or spray-dried porcine plasma (SDPP) diet (same control diet formulation but the Fishmeal LT70 was substituted by 3% SDPP). For each experimental group (control; SDPP group) Total RNA samples were pooled (n= 3 pools each group; n= 4 fish each pool, n= 1 fish taken at random from each tank for each pool) using the same final concentration (133 ng/µl each pool). One-color microarray was carried out according to the manufacturer’s protocols (Agilent Technologies).