Characterization of microRNA signature in activated CD4+ T, polyclonal Th17 (Th17 poly), OVA-specific Th17 (Th17 Ag-sp) cell skewed cultures

To determine specific miRNAs for Th17 cells, we performed comparative miRNA screening of activated CD4+ T, polyclonal Th17 (Th17 poly) and OVA-specific Th17 (Th17 Ag-sp) cell skewed cultures or Th17 Ag-sp sorted cells. The expression of miRNAs analyzed by microarray revealed 27 miRNAs exclusively expressed in culture or sorted Th17 Ag-sp cell subset. Naïve CD4+ T cells from spleen and mesenteric lymph nodes from WT BALB/c mice were cultured in polarizing conditions (TGFbeta/IL-6) for Th17 cells (Th17poly). Cells from OTII mice were skewed for Th17 in the presence of OVA (Th17ag ). Activated CD4+ T cells with anti-CD3/anti-CD28 were used as control. Please note that there are two types of raw data files with two different Grid_Name values 019119_D_20081121 and 019119_D_F_20091030; Raw data files with Grid_Name = 019119_D_20081121 were generated with FeatureExtractor_Version = 10.1.1.1. Raw data files with Grid_Name = 019119_D_F_20091030 were generated with FeatureExtractor_Version = 10.7.3.1.