Square wave voltammetry measurements for electrochemical biosensors

Square wave voltammetry measurements were conducted from 0.4 V to -0.3 V, with frequencies set at 10, 20, and 50 Hz, and pulse sizes 0.001, 0.002, 0.005, 0.01, 0.02, 0.05, 0.1, 0.15, 0.2, and 0.25 V. The experiments were repeated at various step sizes, 0.001, 0.002, 0.005, and 0.01 V. For each experiment 50 ul of the electrolytic liquid was added to the working area of the sensor. After each experiment the sensor was washed three times with deionised water and left to dry before the next experiment was conducted. A concentration of 10 mM ferricyanide and 100 mM KCl in deionised water served as the electrolytic solution.The characterisation with the above-mentioned parameters for three different sensors are given, as well as the post hoc analysis of the frequency and pulse size effects.C-reactive protein was quantified at concentrations ranging from 100 ng/ml to 100 fg/ml with square wave voltammetry, with the resulting measurements being contained in the dataset.

本实验采用方波伏安法对电极进行测量，测试电压范围从0.4 V至-0.3 V，频率设定为10 Hz、20 Hz和50 Hz，脉冲幅度分别为0.001 V、0.002 V、0.005 V、0.01 V、0.02 V、0.05 V、0.1 V、0.15 V、0.2 V和0.25 V。实验在0.001 V、0.002 V、0.005 V和0.01 V的步长下重复进行。每项实验中，向传感器工作区域添加50 μl电解液。每次实验后，传感器用去离子水冲洗三次，并晾干以备下一实验。电解液由10 mM高铁氰化钾和100 mM氯化钾的混合物组成，溶解于去离子水中。对三个不同传感器使用上述参数进行表征，并提供了频率和脉冲幅度效应的后续分析。利用方波伏安法对C反应蛋白进行定量分析，浓度范围从100 ng/ml至100 fg/ml，所得测量数据包含在本数据集中。