Data underlying the publication: Mapping spatial organization of in vitro neuronal networks using high-content imaging

This dataset was generated to study neuronal organization in complete cell culture wells using automated high-content imaging. Primary cortical, hippocampal, and cerebellar cells from mouse brain tissue were plated in 24- or 96-well plates, fixed at 7, 10, or 14 days in vitro (DIV), and stained for neuronal markers. Cortical and hippocampal neurons were labelled with DAPI (nuclei), MAP2 (soma and dendrites), and Ankyrin-G (axon initial segment), while cerebellar Purkinje neurons were identified with Calbindin. Whole wells were imaged via automated confocal microscopy (400 images/well at 10× for 24-well plates; 225 images/well at 20× for 96-well plates). Individual maximum intensity projection images were stitched into full-well reconstructions using an adapted ImageJ plugin.<br>Scripts and code used for generating and analyzing this dataset are available through Zenodo (see at references)

本数据集旨在通过自动化高内涵成像技术，研究完整细胞培养孔内的神经元组织特性。研究对象为从小鼠脑组织分离获得的原代皮层细胞、海马细胞及小脑细胞，将其接种于24孔或96孔培养板中，分别于体外培养第7、10、14天（days in vitro，缩写DIV）进行固定，并采用神经元特异性标志物进行染色。其中，皮层与海马神经元分别以DAPI（标记细胞核）、MAP2（标记胞体与树突）及Ankyrin-G（标记轴突起始段）进行标记，而小脑浦肯野神经元则通过Calbindin完成鉴定。采用自动化共聚焦显微镜对全培养孔进行成像：24孔板每孔采集400张10倍放大图像，96孔板每孔采集225张20倍放大图像。借助经适配改造的ImageJ插件，将单张最大强度投影图像拼接为完整培养孔的重建图像。
本数据集生成与分析所用的脚本及代码可通过Zenodo获取（详见参考文献）