Next Generation Sequencing Facilitates Quantitative Analysis of METTL3 overexpression in THP-1 cells [RNA-seq]

The goals of this study are to compare the downstream m6A modification changes and transcriptional changes in AML cells with or without METTL3 overexpression.mRNA profiles of METTL3-ovexpressing THP-1 cells and of its negative control cells were generated by RNA-seq and m6A-seq, in triplicate, using Illumina Hiseq 2000 METTL3-ovexpressing THP-1 cells and its negative control cells were collected, and the RNA extracted from samples with three biological replicates was subjected to m6A and RNA sequencing. For data analysis, the THP-1 cells were used as reference sample to investigate the role of m6A in AML chemotherapy resistance.