A Conserved Inhibitory Interdomain Interaction Regulates DNA-binding Activities of Hybrid Two-component Systems in Bacteroides

The study reveals a highly conserved inhibitory mechanism to regulate the activities of hybrid two-component systems (HTCSs) in Bacteroides. HTCSs comprise a major class of transcription regulators of polysaccharide utilization genes in Bacteroides. A conserved sequence motif has been discovered to correlate with the interdomain arrangement of HTCS domains. Presence or absence of this motif is likely predictive of the regulatory mechanism evolved for utilization of different glycans.

This dataset includes sequence analyses and structure predictions of HTCSs.

List of files:

·        AlphaFold-HTCS-RR.zip                             AlphaFold results of all HTCS-RR fragments in B. theta

·        AlphaFold-HTCS-cyto-dimer.zip                AlphaFold results of all HTCS-cyto dimers in B. theta

·        HTCSbacteroides-MAFFT-fasta                  Sequence alignment of 6908 HTCSs from Bacteroides

·        HMM-AllHTCS.hmm                                  HMM of HTCSs generated from the MAFFT alignment

·        HMM-DBD-PF12833                                 HMM of HTH18 (Pfam: PF12833) from Interpro

·        HMM-REC-PF00072                                  HMM of REC (Pfam: PF00072) from Interpro

·        B_theta_RR_fasta                                       Sequence alignment of 32 HTCS-RRs in B. theta

·        B_theta_RR-tree                                         A neighbor-joining phylogenetic tree of 32 HTCS-RRs in B. theta