Conidia were inoculated into 200 ml medium at a concentration of 106 conidia/ml, and incubated at 37°C with shaking (200 rpm) for 36 h. The cell wall was extracted as described under Materials and Met
qPCR detection of V. cholerae ATCC 39315 cells in mixed cultures containing 0.5 ng/rx (corresponding to 8x104 GU/rx) of V. mimicus UM 6812. Quantification cycle (Cq) is expressed as mean ± standard de
aRosseti, CA, et. al., manuscript in preparation. bImageJ analysis. (−) gene or protein is down regulated. 16M- Brucella melitensis 16M. N/A- not available.