Processed scRNA-seq Expression Matrix for a TOPK-Targeted NitraTh Vaccine in the GL261 Orthotopic Glioblastoma Model

Project Description:This dataset contains the processed single-cell RNA sequencing (scRNA-seq) gene expression matrices for a preclinical study evaluating a TOPK-targeted NitraTh vaccine in the GL261 orthotopic glioblastoma (GBM) model. The study focuses on characterizing the remodeling of the immunosuppressive tumor microenvironment (TME), specifically within the CD45+ tumor-infiltrating immune cell compartment. Study Design:A total of 3 experimental groups were sequenced. To obtain sufficient CD45+ cells for sequencing, tumor tissues from 6 mice per group were pooled into a single library for each condition (n = 6 mice pooled per group): · Vehicle Group: CpG adjuvant control. · TOPK Group: TOPK protein + CpG adjuvant. · TOPK-NitraTh Group: TOPK-NitraTh fusion protein + CpG adjuvant. Data Content:For each group, we provide the standard filtered feature-barcode matrices generated from the 10x Genomics platform. The .zip archive contains three folders (Vehicle/, TOPK/, TOPK-NitraTh/), each containing: · barcodes.tsv.gz: Cellular barcodes identified in the sample. · features.tsv.gz: List of genes/features (gene IDs and symbols). · matrix.mtx.gz: Sparse matrix containing the counts of unique molecular identifiers (UMIs) for each gene in each cell. Technical Details:The data is ready for downstream analysis using standard bioinformatics tools such as Seurat (R) or Scanpy (Python). All files are fully compressed in GZIP format (.gz) to ensure data integrity and optimize storage efficiency for high-throughput sequencing data. The raw FASTQ sequencing data have been deposited in the NCBI Sequence Read Archive (SRA) under BioProject PRJNA1550462.