Genome-wide analysis of degradation sites in Arabidopsis T87 cultured cell

In a previous study, a high-throughput approach for genome-wide profiling of RNA cleavage sites was developed (e.g. Parallel analysis of RNA end (PARE), 5''P sequencing (5Pseq), Genome-wide mapping of uncapped transcripts (GMUCT)), However, there were some problems with experimental procedures (e.g. Many of the cleavage sites may be mapped in the 3''gene ; It was difficult to accurately identify the multiple cleavage sites in one mRNA). Thus, we performed truncated RNA end sequencing (TREseq) using 3 day after inoculation of Arabidopsis T87 cultured cell to determine the truncated 5'' RNA ends (cleavage sites).