Recognition of CD81/CD9 chimeras by CD9 and CD81 mAbs.

The different chimeras in fusion with EGFP were transfected in Hepa1-6 cells, stained with the indicated mAbs and analyzed by flow-cytometry. The values correspond to the mean fluorescence intensity in the FL1 channel (EGFP) or the FL2 channel, after subtraction of the value obtained with cells labelled only with the secondary reagent. Other mAbs tested did not stain cells expressing any of the chimeras with swaps inside the LEL. These mAbs were TS81, 5A6, Z81, M38 (CD81); SYB-1, TS9, TS9b (CD9). (-): no staining.