Sex Lethal is recruited to chromatin by RNA polymerase III [DamID-seq]

We discovered that the splicing factor Sex lethal (Sxl) also associates strongly with chromatin and that this association is not linked to its RNA-binidng capacity, nor its N- or C-terminal domains. We also found that Sxl is recruited to chromatin by the RNA polymerase III subunt Polr3E. Overall design: Profiling of Sex lethal (Sxl) and Polr3E DNA binding in larval neurons of the Drosophila brain. Also includes binding of Sxl mutants and Sxl in a Polr3E RNAi background.