Single cell transcriptome analysis of decidua macrophages in normal and recurrent spontaneous abortion patients. Single cell transcriptome analysis of decidua macrophages in normal and recurrent spontaneous abortion patients

We isolated CD14+CD45+ dMΦ from the normal or RSA decidua by flow cytometry, followed by single cell RNA sequencing (scRNA-seq). In total, 23,062 single-cell transcriptomes of macrophage were profiled (12,470 Normal and 10,592 RSA), which were divided into 13 major clusters via T-distributed stochastic neighbor embedding (t-SNE) visualization. Overall design: Our study redefined the polarization state and physiological characteristic of dMΦ in early normal and RSA pregnancy