Transcriptomic Characterization of Human Beige Adipocyte Differentiated From Adipose Mesenchymal Stem Cells

Beige adipocytes are a distinct type of thermogenic fat cells in human. Since beige adipocytes are distributed sporadically within white adipose, characterization of human beige adipocytes has long been a problem. In this study, we reported a rapid and roboust method in generating human beige adipocyte with chemically defined medium and RNA-Seq was perfomed to reveal the molecular characterization of derived human beige adipocytes. Overall design: mRNA profiling of adipose derived mesenchymal stem cells and in vitro differentiated beige adipocytes were generated by RNA-Seq, in duplicate, using illumina NextSeq platform. mRNA profile of in vitro differentiated white adipocytes were also generated by same method and serve as control.