RNAseq on developmental endoreplicating (salivary glands) cells in Drosophila melanogaster

In order to evaluate the global transcriptomic changes in endoreplicating cells in Drosophila melanogaster we performed RNA-Seq. Endoreplicating cells (salivary glands) and mitotic cycling cells (brains and imaginal discs) were harvested from feeding 3rd instar larvae and mRNA levels were compared. Consistent with our previous results, we found that endoreplicating cells repress genes regulated by the E2F1 transcription factor as well as genes regulated by the Myb-MuvB (MMB) transcription factor complex. Brains and imaginal discs (BD) and Salivary Glands (SG) were dissected from feeding 3rd instar y1 w67c23 larvae and total RNA was isolated. After RNA isolation, poly-A tailed RNA was enriched and cDNA libraries were generated. RNAseq was done on three biological replicates using an Illumina NextSeq 500.