Numbers of putative α-Syn epitopes for presentation to T cell receptors predicted from the binding potential of MHC class I and II molecules for the aa sequence of α-Syn.

Number of predicted T-cell epitopes from the 38-mer C-terminal fragment of α-Syn are in brackets. aFor class I MHC binders three following grades for scoring included: low immunogenic epitopes, scores <−3; mild immunogenic epitopes, scores >−3 but <−2; high immunogenic, scores >−2; all computations were done using the Immune Epitope Database and Analysis Resource (IEDB) (http://immuneepitope.org) and integrative epitope prediction tool [proteasomes cleavage, Transporter associated with Antigen Processing (TAP) binding, processing and MHC binding]. bFor class II MHC binders, scoring grades were based on predicted IC50 values and were: low (1000 nM–5000 nM), intermediate (200–1000 nM) and high (<200 nM) for groove binding prediction by MHCPred. This prediction algorithm considers peptides with predicted IC50 >5000 nM as non-binders [86], [87].