ASSEMBLY: Bacteriophages Roam the Wheat Phyllosphere

Dataset description This record contains seven FASTA files associated with the article “Bacteriophages Roam the Wheat Phyllosphere.” The assemblies and derived viral sequence sets were generated from wheat phyllosphere viral metagenomes used to characterize bacteriophage diversity associated with winter wheat leaves. The dataset includes assemblies from flag leaf (FL) and penultimate leaf (PL) samples, both from native and multiple displacement amplification (MDA) virome preparations, as well as predicted viral contigs, representative virus operational taxonomic units (vOTUs), and two manually recovered complete viral genomes. The dataset includes: FL_amp_spades.fasta: amplified flag leaf virome assembly generated with SPAdes/metaSPAdes; 3,188 contigs. FL_spades.fasta: flag leaf virome assembly generated with SPAdes/metaSPAdes; 6,564 contigs. PL_amp_spades.fasta: amplified penultimate leaf virome assembly generated with SPAdes/metaSPAdes; 4,393 contigs. PL_spades.fasta: penultimate leaf virome assembly generated with SPAdes/metaSPAdes; 11,830 contigs. rmanually_recovered_complete_viral_genomes.fasta: two complete viral genomes manually recovered from subsampled assemblies: NODE_40, recovered from the 80% subsampling of PL, and APSE_wheat, recovered from the 10% subsampling of PL_mda; 2 complete viral genomes. viral_contigs.fasta: predicted viral contigs recovered from the wheat phyllosphere virome assemblies; 15,739 contigs. vOTUs.fasta: representative virus operational taxonomic units after dereplication/clustering; 876 vOTUs. Together, these files allow comparison of viral contigs recovered from wheat phyllosphere viromes across leaf types and amplification conditions. They support analyses of bacteriophage diversity, viral operational taxonomic units, phyllosphere-associated viral novelty, manually recovered complete viral genomes, and bacteriophage community structure in the wheat phyllosphere. Recommended citation Forero-Junco, L. M., Alanin, K. W. S., Djurhuus, A. M., Kot, W., Gobbi, A., & Hansen, L. H. (2022). Bacteriophages Roam the Wheat Phyllosphere. Viruses, 14(2), 244. https://doi.org/10.3390/v14020244