Targeting Plk1 re-establishes the response to immune checkpoint therapy in pancreatic cancer

Purpose: Standard RNA-Seq analysis using Plk1 knock-in mouse model. Overall design: The Plk1 knock-in mouse (Plk1LSL) was designed by the Xiaoqi Liu lab at Purdue University, and constructed by Taconic, Germany. Plk1LSL mice were subsequently obtained from these recombinant ES cells. Mist1Cre/+/KrasG12D/+ mice were generated by the Konieczny lab at Purdue University, whereas Rosa26CreERt2 mice were purchased from the Jackson Laboratory. To generate Mist1CreER/+/Plk1LSL mice, we crossed the Plk1LSL line to Mist1CreER/+ line. To generate Mist1CreER/+/KrasG12D/+/Plk1LSL mice, we crossed the Mist1CreER/+/KrasG12D/+ line to the Plk1LSL line. To generate Rosa26CreERt2/Plk1LSL mice, we crossed the Plk1LSL line to Rosa26CreERt2 line. All procedures involving mice were guided by Purdue University Animal Care and Use Committee (Protocol no: 1111000133E001) and University of Kentucky Animal Care and Use Committee (Protocol no: 2020-3685).