Protein–protein interactions (PPIs) have been extensively utilized in synthetic biology to construct artificial gene networks. However, synthetic regulation of gene expression by PPIs in E. coli has l
GoldenPiCS modules and plasmids. Modules and plasmids are listed with corresponding cloning- and fusion sites and full sequences. DNA orientation is 5’to 3′. All plasmids are available at Addgene. (XL
pSHDY# shuttle plasmid backbone, version w/o mobA Y25F point mutation. Precursor backbone of pSHDY as described by Behle et al., 2019 (https://doi.org/10.1101/757948).
