Coelomocyte culture media evaluated.

1aCF, artificial coelomic fluid; MB, marine broth; MEM, Dulbecco’s Modified Eagle Medium; CCM, coelomocyte culture medium, CF, coelomic fluid, ECCM, echinoid coelomocyte culture medium.*per suppliers instructions; Gibco™, Invitrogen, Carlsbad, CA.**200 U/ml Penicillin G sodium salt, 200 µg/ml Streptomycin sulfate and 25 µg/ml Ampicillin sodium salt.***Coelomocytes detached from culture well surface, formed small aggregates and floated in medium after ON (16–21 hr) incubation.Bolded text indicates components not present in the original cell culture recipe. Other modifications to the original recipe included the omission transferrin, selenous acid and chemically defined lipids (CDL), as well as growth factors, including epidermal growth factor (EGF) and basic fibroblast growth factor (b-FGF).