Characterization of the recovered viruses.

Replication of recovered viruses was assessed using a combination of several criteria: (i) the presence or absence of cytopathic effect (CPE) is highlighted in green or red, respectively, (ii) the amount of viral RNAs in cell supernatant at the second passage, assessed using a real-time RT-PCR assay is reported as mean Log10 copies/mL ±SD (iii) the infectious titer of each of the rescued virus at the second passage is expressed as a mean log10 TCID50/mL ±SD.