scATAC-seq of innate immune cells from mice treated with the adjuvant 3M052

Purpose: to explore the epigenomic landscape of innate immune cells stimulated with a novel adjuvant, 3M052 Methods: Mice were injected with 3M052. Draining lymph nodes were negatively selected for CD19+ and CD3+, then flow sorted into four populations: Dendritic cells (DCs), Double positive cells (DP, CD11b+BST1+), Ly6c+ cells (Ly6c), and plasmacytoid dendritic cells (pDCs). Lymph nodes were harvested at baseline (D0), 24 hours post-treatment (D1) or 28 days post-treatment (D28). single cell ATAC seq of innate immune cells in the lymph nodes of mice after treatment with 3M052