Comparative 16S rRNA V3-V4 amplicon sequencing of gut microbiota from fluoride-exposed and control population

This study examines gut bacteria in fluoride-exposed asymptomatic subjects, exposed symptomatic subjects, and unexposed control. Stool samples were collected, gut bacteria were isolated, and cultured isolates were pooled. DNA was extracted and processed using 16S rRNA V3-V4 amplicon sequencing to investigate microbial community patterns. The resulting dataset may support research exploring potential associations between fluoride exposure, gut microbiome structure, and skeletal fluorosis.