Transcriptome analysis of freshly isolated young and aged mouse natural regulatory T cells

Remyelination efficiency declines wiith aged leading to axonal loss and irreversible disability. Regulatory T cells have been previously shown Young and aged natural Tregs were isolated from spleen and lymphnodes of C57Bl6/J mice. In brief, spleen and lymph nodes were mashed through a 70µm strainer and red blood cells lysed. Natural Tregs were then magnetically isolated using CD4 negative selection (Stemcell technologies) followed by CD25 positive selection (Stemcell technologies). CD25+ cells were then lysed with Trizol and frozen at -80C. young and aged natural Tregs were always isolated simultaneosly and once all samples were obtained, RNA was isolated.