circRNA and mRNA transcriptome sequencing data from OVA-induced mouse lung tissue

<b>Chronic Allergic Pulmonary Inflammation Model</b>The male C57BL/6J mice utilized in this study were six weeks old, weighed 22±2 grams. The mice were allocated into four distinct groups, each consisting of six animals: the control group, the asthma group, the exercise group, and the asthma + exercise group. The asthma group and asthma+ exercise group were administered aluminum hydroxide-diluted OVA at a dose of 10 μg per mouse via intraperitoneal injection on days 0, 14, 28, and 42, following a previously established protocol. From day 21 to day 54, the mice in these groups were exposed to a 1% OVA aerosol for 30 minutes, three times per week. In contrast, the mice in the control group and the exercise group were exposed to saline under the same conditions. Mice are anesthetized with 1% pentobarbital sodium via intraperitoneal injection. Euthanasia is performed by tail vein injection of a 3% pentobarbital sodium solution.<b>RNA Sequencing</b>Shanghai Biotechnology Corporation conducted the RNA microarray analysis. Total RNA was extracted from the lung tissues of three OVA-induced mice and three healthy control mice via the RNeasy Mini Kit (Qiagen). For RNA-seq, strand-specific libraries were prepared via the TruSeq Stranded Total RNA Sample Preparation Kit (Illumina). the samples were quantified via a Qubit 2.0 fluorometer (Life Technologies), and the insert size was verified via a 2100 Bioanalyzer (Agilent). Following the dilution of the library to 10 pM, clustering was achieved via cBot, and sequencing was conducted on the Illumina NovaSeq 6000 platform. Shanghai Biotechnology Corporation was responsible for both library construction and sequencing.