hnRNPK is involved in mRNA processing of a significant fraction of the transcriptome. Mus musculus

Here, we report the first genomic-scale comprehensive characterization of mouse transcripts bound to hnRNPK. hnRNPK binding at the isoform level was evaluated via RNA-sequencing of liver samples obtained from obese and wild-type mouse models. The comparison of hnRNPK co-immunoprecipitated fractions with total RNA fractions in a given mouse model enabled quantitative evaluation of the binding affinity of hnRNPK for thousands of isoforms. Overall design: Total RNA, cytoplasmic fraction and nuclear fraction was isolated from liver of three mouse strains (wild type-normal diet, wild type-high fat diet, leptin defficient-normal diet). Cytoplasmic and nuclear fractions were subjected to immunoprecipitation with anti-hnRNPK antibody (and IgG as control). Comparison of immunoprecipitated and total fraction allowed for estimation of extent of hnRNPK-mediated translational regulation.