Gene expression profile at single cell level of pre-cDCs from the bone marrow, spleen and lung of mice

Type 2 pre-cDCs are considered as a homogeneous population in the bone marrow. We used single cell RNA sequencing to show that the bone marrow contains two subsets of pre-cDC2s that are committed to give rise to different different cDC2 subsets in peripheral tissues. pre-cDCs from the bone marrow, spleen and lung of WT C57BL6 mice were isolated by Fluorescence-activated cell sorting (FACS) and analyzed using scRNAseq.