Confirmation of direct interaction between H3K56cr and GLYR1 by H3K*56cr Site-Link in living cells

As we have found that H3K56cr can crosslink with GLYR1 in living cells, we now perform H3K*cr Site-Link in GLYR1 overexpressed cells. The subsequent 365-nm light irradiation could activate the diazirine and covalently capture potential effector proteins on native chromatin in living cells. The crosslinked band could be identified after gel-based proteomics and pLink assisted MS/MS analysis of the crosslinked peptide.