figure.rar

Prolonged culturing in vitro often
leads to accumulation of genetic changes in cells. Although human multipotent mesenchymal stromal cells (hMSC) are considered as
genetically stable, there is a risk of genomic instability and
structural chromosome instability and, therefore, side effects of cell therapy
associated with long-term effects. In this study, the karyotype, genetic
variability and clone formation analyses have been carried out in the long-term
culture MSC from human gingival mucosa.

The immunophenotype and STR loci combination
did not change during the process of cultivation. From passage 23 the proliferative
activity of cultured MSCs was significantly reduced. From passage 12 of
cultivation, clones of cells with stable chromosome aberrations have been
identified and some of these (12%) are tetrasomy of chromosome 8. The random genetic
and structural chromosomal aberrations and the spontaneous level of chromosomal
aberrations in the hMSC long-term cultures were also described.