Cytotoxic activity of recombinant barnase and scFv 4D5-dibarnase proteins1.

ND, not determined; -, not reached1Cell viability assays were performed as described in Materials and Methods. The ICnn is the concentration that results in nn% reduction of cell viability after 72 h of incubation with protein.2All the cells, except for CTLL-2, are of human origin; cell lines marked by asterisk were from American Type Cell Collection, the others were from cell collection of Department of Immunology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russia.3Cell lines overexpressing HER2.4hPBMCs were isolated from the peripheral blood of healthy donors.5Types of myeloid leukemia cell lines are termed according to [60].