Transcriptome differences between primary brown adipocytes of wildtype and UCP1-KO mice. Transcriptome differences between primary brown adipocytes of wildtype and UCP1-KO mice

We analyzed coding transcript abundance in fully differentiated, primary brown adipocytes from murine, interscapular brown adipose tissue of wildtype and UCP1-KO mice. Overall design: We prepared primary cells from the stromal-vascular fraction of murine interscapular brown fat and differentiated these in vitro into brown adipocytes. Three independent cultures each were generated from wildtype 129SvEvS1 mice and UCP1-KO mice on the same strain background. We sequenced total RNA and determined transcript abundance.