In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. This platform profiles
Additional file 2: Dataset S1. Excel workbook containing all yeast two-hybrid prey sequences from colonies with single prey plasmid copies. Each row represents the result from a single colony and incl
a Intensities were measured using fraction 10 of Fig 4A for wild-type Rpo41 and fractionbFree_Rpo41 = Iupper—[Ilower (MWRpo41 /MWMtf 1)]cBound_Rpo41 = Iupper—Free_Rpo41Densitometry scanning of SDS wit