Lacrimal Gland Gene expression in Aromatase Knockout Mice and Wild Type Controls

The Effect of Aromatase Knockout on Gene Expression in the Mouse Lacrimal and Meibomoan Gland. Keywords: Aromatase Knockout vs Wild Type Control Lacrimal and meibomian glands were harvested from homozygous male and female aromatase knockout mice and age matched wild type controls. Tissues were pooled into 3 biological replicates and were hybridized to separate microarrays. Each cRNA prep was hybridized to a GE Healthcare/Amersham Biosciences CodeLink UniSet Mouse 20K I Bioarray and a Affymetrix GeneChip Mouse Expression Array 430A.芳香化酶敲除对小鼠泪腺与睑板腺基因表达的影响。关键词：芳香化酶敲除（Aromatase Knockout）与野生型对照（Wild Type Control）。本研究从纯合子雌雄芳香化酶敲除小鼠及年龄匹配的野生型对照小鼠体内采集泪腺与睑板腺组织。将采集的组织混合为3份生物学重复样本，分别进行微阵列杂交。每份cRNA制备样本分别与GE医疗/安玛西亚生物科学（GE Healthcare/Amersham Biosciences）的CodeLink UniSet小鼠20K I生物芯片，以及Affymetrix GeneChip小鼠表达阵列430A进行杂交。