Data from: Self-assembled nanoclay gel with spheroid MSC-derived exosome mimetics to integrate demineralized bone matrix and noggin-targeting miRNA for synergistic osteogenesis

Demineralized bone matrix (DBM) is widely used as an alternative to
autografts for repairing bone defects, but its clinical efficacy is
limited by poor retention at the defect site and inadequate osteogenic
capacity. Here, we developed a multifunctional carrier system aimed at
improving the capacity of DBM by utilizing nanoclay-based self-assembly
along with cell-derived exosome mimetics (EMs). EMs were derived from
mesenchymal stem cells (MSCs) osteogenically induced in spheroids and were
functionalized with bisphosphonates (BP) to assemble a stable gel network
with laponite nanoclays via BP-nanoclay edge interactions. The resulting
self-assembled nanoclay gel exhibited excellent injectability,
moldability, and self-healing properties, even when loaded with a high
concentration of DBM, and supported MSC osteogenic differentiation. We
further enhanced the potency of DBM-mediated osteogenesis and bone
morphogenetic protein (BMP) signaling in the nanoclay gel by incorporating
BP-functionalized EMs loaded with miR-200c that can target BMP antagonist
noggin. Lastly, we validated the bone regeneration efficacy of DBM-loaded
nanoclay gels in comparison to commercially available DBM putties using a
mouse calvarial defect model. This approach presents a versatile nanoclay
gel carrier platform that overcomes the limitations of current DBM
formulations and provides a promising strategy for improved bone repair.