7-AMC adducts detected in <i>wt B. cereus</i> conditioned supernatants.
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Wild type B. cereus 569 spores were resuspended in 200 µl TMB buffer to OD580 = 1. Spores were treated with 0.2 mM inosine and supernatants were collected 30 min post-inosine addition. Collected supernatants were labeled with 7-AMC. 7-AMC adducts sere separated by RP-HPLC and identified by mass spectrometry. Concentrations were calculated by fluorescence spectroscopy.
创建时间：
2013-02-21
