S1P content in lysate and conditioned medium of [3H]serine pulsed MSCs.
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[3H]lipids were extracted by MSCs cultured in the presence or in the absence (control) of 5 µM sphingosine kinase inhibitor (iSK) and successively pulsed with [3H]serine for 2 h.[3H]lipids were separated by TLC as described in Materials and Methods Section.S1P content in lysate and conditioned medium of [3H]serine pulsed MSCs.
创建时间：
2015-12-02
