Single-cell and bulk RNA sequencing of mouse atherosclerosis disease stage course [adipose_liver_bulkRNA]

In addition to the pathological processes in the vascular wall, atherosclerotic coronary artery disease (CAD) development is affected other tissues, including metabolic tissues such as liver and white adipose tissue (WAT). However, the transcriptional events occurring in metabolic tissues in response to pro-atherosclerotic conditions (hypercholesterolemia and obesogenic diet), remain incompletely understood. Here, we profile liver and WAT gene expression in mouse at increasing exposure levels of atherogenic factors. A disease stage course of atherosclerosis in mouse was established by varying genotype (wild type and proatherogenic LDLR-/- ApoB100/100) and diet (0, 1 or 3 months of high-fat diet), followed by bulk RNA-Seq of liver and perigonadal adipose tissue (epididymal in male and periovarial in female).