TCF7L1 controls tuft cell differentiation

To study the functions of Tcf7l1 during gut development, we have ablated Tcf7l1 specifically in the intestinal epithelium using Shh-Cre. To determine transcriptional changes upon loss of Tcf7l1, we have isolated EpCAM-positive epithelial cells from mouse embryos using fluorescence activated cell sorting (FACS) and performed RNA-sequencing analysis.为研究Tcf7l1在肠道发育过程中的功能，我们利用Shh-Cre特异性敲除了肠上皮细胞中的Tcf7l1。为探明Tcf7l1缺失引发的转录组变化，我们通过荧光激活细胞分选（FACS）从小鼠胚胎中分离出上皮细胞黏附分子（EpCAM）阳性的上皮细胞，并开展了RNA测序（RNA-sequencing）分析。