Single nucleus RNA sequencing reveals heterogeneity among multiple white adipose tissue depots

Regardless of its anatomical site, adipose tissue shares a common energy-storage role but exhibits distinctive properties. Exploring the cellular and molecular heterogeneity of white adipose tissue (WAT) is crucial for comprehending its function and properties. In this study, we employed Single nucleus RNA sequencing (snRNA-seq) to test five representative depots including inguinal, epididymal, mesenteric, perirenal, and pericardial adipose tissues in mice under physiological conditions. By analyzing the contents of main cell categories and gene profiles of various depots, we identified their distinctive physiological properties. To construct the atlas of cell types in different adipose tissue depots, we performed snRNAseq of epididymal white adipose tissue (EWAT), subcutaneous white adipose tissue (SWAT), mesenteric white adipose tissue (MWAT), perirenal white adipose tissue (NWAT) and peri-heart white adipose tissue (HWAT) pooled from mice fed chow diet for analysis.无论解剖部位如何，脂肪组织均具备共同的能量储存功能，但同时展现出各异的特性。探究白色脂肪组织（white adipose tissue, WAT）的细胞与分子异质性，对于理解其功能与特性至关重要。本研究采用单细胞核RNA测序（single nucleus RNA sequencing, snRNA-seq）技术，对生理状态下小鼠的5处代表性脂肪垫——腹股沟、附睾、肠系膜、肾周及心包脂肪组织——进行了分析。通过分析各脂肪垫的主要细胞类群构成与基因表达谱，本研究明确了它们各自独特的生理特性。为构建不同脂肪垫的细胞类型图谱，本研究对取自饲喂常规饲料小鼠的附睾白色脂肪组织（EWAT）、皮下白色脂肪组织（SWAT）、肠系膜白色脂肪组织（MWAT）、肾周白色脂肪组织（NWAT）及心周白色脂肪组织（HWAT）的混合样本开展了单细胞核RNA测序分析。