Single cell transcriptomic analysis of the lateral hypothalamic area reveals molecularly distinct populations of inhibitory and excitatory neurons

We employed a droplet-based single cell RNA-sequencing (scRNA-seq) approach to develop a comprehensive census of molecularly distinct cell types in the mouse lateral hypothalamic area (LHA). In addition to 13 distinct non-neuronal cell populations, we define 15 distinct populations of glutamatergic and 15 distinct populations of GABAergic neurons, including both known and novel cell types. This comprehensive transcriptomic analysis of LHA cell types lays the groundwork for understanding the circuit-level underpinnings of LHA coordination of innate behavior. Two single cell transcriptomic (Chromium v2 chemistry) data sets of P30-aged mouse LHA, one of 3 pooled male and the other of 2 pooled female animals.本研究采用基于液滴的单细胞RNA测序（single cell RNA-sequencing, scRNA-seq）技术，对小鼠下丘脑外侧区（lateral hypothalamic area, LHA）内具有独特分子特征的细胞类型开展了全面的鉴定与普查。本研究共鉴定出13种独立的非神经元细胞群，同时明确了15种谷氨酸能神经元与15种γ-氨基丁酸能神经元细胞群，涵盖已知及全新的细胞类型。本次针对LHA细胞类型的全面转录组学分析，为解析LHA协调先天行为的环路层面机制奠定了重要研究基础。本数据集包含两份基于P30龄小鼠LHA的单细胞转录组（Chromium v2 chemistry）数据，分别来自3只混合的雄性小鼠与2只混合的雌性小鼠。