This file contains additional information on Materials & Methods used in this study and expanded Results.

The Materials and Methods include; a) method for the determination of optimum endothelial vasodilatation by pGz in mice, b) methods for the determination of the time course of protein expression and phosphorylation of eNOS and Akt, c) methods for the determination of ROS in cardiomyocytes, d) supplemental description for methods for protein expression and phosphorylation, e) method for animal euthanasia. The Results section includes; a) representative tracing of the aortic pulse waveform in mice and the effects of pGz of varying frequency on the position of the dicrotic notch, b) figures for the effect of pGz frequency on mean arterial blood pressure and the change in a/b ratio, c) figures on the effects of duration of pGz on protein expression of eNOS and the ratio of p-eNOS/eNOS, d) figures on the effects of pGz on genomic upregulation of eNOS, e) figures on the effects of duration of pGz on protein expression of Akt and p-Akt/Akt, f) figures on the effect of 4 weeks of pGz on eNOS and p-eNOS protein expression in control (wt) and Duchenne Muscular Dystrophy (mdx) mouse model.
(DOCX)