Effect of low protein diet on parenchymal tumor associated macrophages (TAMs) and stromal mammary tissue macrophages (MTMs) in control-PyMT and MYC overexpressing PyMT ( S100a8-creRosa26-LSL-MYCPyMT ) tumor

To study the low protein diet and cancer genetic effect on the macrophage in tumor, we treated the control-PyMT and S100a8-creRosa26-LSL-MYCPyMT (MYC-PyMT) mice reconstituted with wt bone marrow with low protein diet for 6 weeks Overall design: Compare the gene expression between TAMs and MTMs isolated by FACS from tumor of control-PyMT and MYC-PyMT mice treated with low protein diet