Data for filmpaper.docx

<b>Background</b>: The escalating demand for eco-friendly alternatives in nanoparticle synthesis has spurred the exploration of plant-based approaches, offering promising applications across various biomedical domains. This study delved into the synthesis of silver nanoparticles (AgNPs) utilizing <i>Aloe vera </i><i>(L.) Burm.f.</i> extract and subsequently investigated their potential to enhance the antimicrobial properties of Eri silk fibroin Films (ESF). The objective was to develop biodegradable antimicrobial wound dressing materials. Silk fibroin, a natural biopolymer derived from Eri silkworm (<i>Samia cynthia ricini </i>Donovan) cocoons, exhibits inherent antimicrobial characteristics. By synergistically integrating AgNPs into ESF, we sought to augment its antimicrobial efficacy, providing a versatile and sustainable solution for wound healing and infection prevention.<b>Methods</b>: The green synthesis of AgNPs involved using <i>A. vera</i> extract to reduce silver nitrate (AgNO₃). The nanoparticles were characterized using various techniques including FTIR, UV-visible spectroscopy, and SEM with EDX analysis. Uniformly sized (0.42 cm²) films were prepared in six different concentrations of blended Eri silk fibroin Films (ESF) with <i>A. vera</i> Silver Nanoparticles (AVAg). These were compared against non-blended ESF as a control and a commercially available, non-medicated cotton gauze (Surgicom®) as a positive control. The antimicrobial efficacy was tested against common human pathogens such as<i> Escherichia coli, Staphylococcus aureus, </i>and<i> Candida albicans</i>.<b>Results</b>: The blended ESF films displayed enhanced water absorbability (81.5%-91% after 96 h), porosity (78.38%-88.46%), and a water vapor transmission rate of 2503.19 g/m²/day, demonstrating their potential as wound dressings. SEM analysis showed a strong presence of silver on the fibrous matrix, with EDX revealing a high silver composition (78.66±0.70%). In vitro antimicrobial assays indicated substantial efficacy against <i>E. coli</i>, <i>S. aureus</i>, and <i>C. albicans</i>, highlighting the promising role of AgNPs in combating antibiotic resistance.<b>Conclusion</b>: The <i>A. vera</i>-assisted green synthesis of AgNPs is a sustainable and effective method for producing stable nanoparticles. When incorporated into silk fibroin films, these nanoparticles enhance the material’s physical and antimicrobial properties, making it suitable for biomedical applications, particularly wound healing and infection control.