five

Affinity_enhancement_Boskar4

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NIAID Data Ecosystem2026-05-02 收录
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Affinity maturation of Boskar4 to increase its biological activity as a G-CSFR agonist. Therefore, two libraries (a saturation and a design library) were created and screened with an E. coli display system and FACS. Conclusion: The expected shift to higher fluorescence values over the course of the enrichment sorts (5 in total) was observed, and later a total of 16 affinity-enhanced unique variants were extracted from these FACS-enriched pools after 3 and 5 sorts.
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2024-10-01
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