Single cell RNA sequencing of polarized cystic fibrosis (CF) and non-CF human airway cell cultures following transduction of primary basal cells with lentivirus

The goal of this study was to compare cell composition, gene expression, and transduction efficiency of different epithelial cell types following transduction with lentiviruses containing either green fluorescent protein (GFP) or the human CFTR gene. Primary basal cells were collected from CF and non-CF lung donors, transduced with a lentivirus and polarized, prepared for single cell sequencing via 10X Genomics Chromium controller, and sequenced on Illumina HiSeq4000 and NovaSeq6000