Loss of function of MALAT1 on primary human OA osteoblast transcriptome

Cells: Primary human osteoblasts from subchondral bone of osteoarthritis patients (n=3 patients). Treatments: Cells transfected using Lipofectamine 3000 (Qiagen) with either control LNA (NC) or MALAT1 LNA1 or MALAT1 LNA2 for 24 hours. Total RNA extracted by Qiagen RNeasy columns. RIN values >7 via Agilent Bioanalyser. Analysis: RNA subjected to Quantseq 3' RNA sequencing (Lexogen) and sequenced reads mapped to the hg38 reference human genome. Overall design: 9 samples analysed in total. Primary OA subchondral bone osteoblasts from n=3 patients transfected for 24h with either (i) LNA control, (ii) MALAT1 LNA1 or (iii) MALAT1 LNA2.