The molar ratios of DOPG/[Ca²⁺] and (DOPG+MO)/[Ca²⁺] after rapidly mixing the DOPG/MO-based vesicles with PIPES buffer (pH 7.0) containing 68 mM Ca²⁺ ions.

The investigated vesicles in the absence of Ca2+ ions were composed of DOPG and MO at a constant molar ratio of 30∶70 and the total lipid content was 7 wt%. Eight different rapid-mixing investigations with different volume ratios of the vesicles (volume A) to the buffer (volume B) are summarized. In addition, the final Ca2+ concentrations are given.