Single cell RNA sequencing of the adult female mouse urethra with transurethral instillation of Lipopolysaccharide (LPS) compared to PBS instilled mice

We have employed single cell RNA-sequencing to create a cellular atlas of the female mouse urethra. The epithelial lining of the urethra is an understudied mucosal barrier. Emerging evidence points to the role of the urethra in pathogen surveillance, sensation and response. In this study, we instilled female mice transurethrally with PBS or LPS (1 mg/ml) and collected urethras at 3 hours post-instillation. Single cell RNA-sequencing was performing to assess changes in immune and epithelial cell types after LPS treatment. Overall design: Wildtype CD-1 adult female mice were transurethrally instilled with PBS (100 microliter) or bacterial lipopolysaccharide (LPS, 1 mg/ml, 100 microliter). Urethras were dissected 3 hours post-instillation and digested to obtained single cells. Three mice each were pooled for the PBS and LPS group. Single cell RNA-sequencing was performed using 10x Genomics 3' gene expression kit.