d8429535-2996-4469-a85f-cb12bda3649d - samples
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https://www.omicsdi.org/dataset/ega/EGAD00001009831
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资源简介：
Sorted single CD8+T cells expressing CD14 from human liver for SMARTSeq2.
Livers processed: Kucykowicz et al STAR Prot 2022:pubmed.ncbi.nlm.nih.gov/35516846/
Published: Pallett et al Nature 2022
Tissue CD14+CD8+T-cells are reprogrammed by myeloid cells and modulated by LPS
A modified SMART-seq2 protocol was performed on the single flow cytometry sorted-cells as previously described58. After cDNA generation, libraries were prepared (384 cells per library) using the Illumina Nextera XT kit (Illumina). Each library was sequenced to achieve a minimum depth of 1-2 million raw reads per cell using an Illumina HiSeq 4000 using v. 4 SBS chemistry to generate 75-bp paired-end reads.EGA dataset EGAD00001009831
创建时间：
2023-01-03
